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The small creatures - Insects, Invertebrates and bugs

India sanjay Offline
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( This post was last modified: 01-11-2017, 08:18 PM by sanjay )

For time being, In this thread we are going to post information, images and videos related to small creatures. They are very very special and show behaviors which resemblance to aliens.
I request members to post interesting information here.
I am starting with a video that I saw in FB, It is related to Mantis, showing a parasite living inside it.

Its video, click to play it



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genao87 Offline
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what kind of parasite is that??
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Italy Ngala Offline
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(08-03-2017, 06:16 AM)genao87 Wrote: what kind of parasite is that??

Is a parasite worm of the Nematomorpha phylum, famous for parasitizing grasshopper and cricket (Orthoptera); he pushes the guest to go into the water, where he will certainly drown and then die, and the mature parasite go out the victim and so will can may reproduce.

For other information you can search Spinochordodes tellinii and Paragordius tricuspidatus.
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Argentina Tshokwane Offline
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Credits to Gil Wizen.

Rhynchotermes – the best of both worlds.

Termites are truly unique because they are among the few hemimetabolous insects (lacking the pupal stage in their life cycle) to develop an eusocial lifestyle, with different reproductive castes, division of labor, and overlapping generations. In stark contrast to eusocial Hymenoptera (ants, bees, and wasps), termite colonies follow a different structure, often with a single long-lived royal pair responsible for egg production (as opposed to male Hymenoptera that die soon after mating), but also include a secondary reproductive caste. Workers and soldiers can be both males and females (in Hymenoptera – all females). From an ecosystem standpoint, termites play a vital role as detrivores, feeding on and breaking down dead plant tissue and wood. For this reason they rely on gut symbionts (protozoans, bacteria, and flagellates) that assist in breaking down cellulose.


One of the things you often learn about termites in an entomology course is that there are two types, easily distinguished by their soldiers: species with mandibulate soldiers (possessing jaws), and species with nasute soldiers (with a long nose). The mandibulate soldiers use their enlarged strong mandibles to physically attack and injure intruders. They cannot use their jaws for feeding, and are therefore dependent on mouth-to-mouth feeding from the workers. In contrast, the nasutes deploy chemical defense by secreting various compounds from their nose, mainly to use as deterrents against ants, but also with some effect over much larger predators such as tamanduas.

Why this long introduction? As things usually go in nature, and more specifically in arthropods, to every rule there is an exception. Last year I travelled to Costa Rica, and one of the species I was hoping to find was a very unique termite.

Armed nasute termite soldier (Rhynchotermes perarmatus)

*This image is copyright of its original author

This monstrous beast is a soldier of Rhynchotermes perarmatus, a nasutiform termite. However, contrary to the “rule” I mentioned above, soldiers of this species possess both a chemically armed snout and well developed mandibles. They are now treated by taxonomists as being mandibulate nasute.


The neotropical genus Rhynchotermes contains several species, all have nasute soldiers with noticeable mandibles. However, only in two species the mandibles are massive – Rhynchotermes perarmatus and R. bulbinasus.

*This image is copyright of its original author

Rhynchotermes perarmatus is subterranean, nesting underground or under stones. These termites usually do not expose themselves to the outside world, but instead move inside covered tunnels constructed from soil particles. Inside these dark tunnels the stout workers run clumsily, carrying debris and compressed wood fiber back to the colony for food.

An intimate look at Rhynchotermes perarmatus termites crawling in one of their covered nest tunnels

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An active tunnel contains a thick flow of worker termites, and several soldiers scattered at the periphery, on guard.

*This image is copyright of its original author

Rhynchotermes seems to be associated with slightly disturbed habitats, such as cleared forest areas or meadows used for cattle grazing. There are reposts of them active under aged dried out cattle dung, suggesting they may have a role in breaking it down and recycling the nutrients. In Costa Rica I found Rhynchotermes perarmatus under a heavily decomposed fallen tree, right besides a well-maintained trail. Still, after flipping the log I could not see them. I had to break open one of the galleries to get access to the action.

And the soldiers did not like that.


*This image is copyright of its original author

While the workers kept on running seemingly undisturbed, the armed soldiers started pouring out, seeking the intruder. Maybe this is the time to mention that termite soldiers are usually blind. They have no functional eyes, and rely on chemical cues and physical proximity for defending the colony.

*This image is copyright of its original author

Even tough beetles like this weevil know to steer clear of active Rhynchotermes perarmatus soldiers.

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To the human eye it seems like despite their menacing appearance, Rhynchotermes perarmatus soldiers do not do much. They walk around aimlessly, then suddenly rise on their feet and give a mute roar, gaping their mandibles. But what seems harmless to us is actually a well thought of strategy: the soldier’s head contains a special gland that secretes a cocktail of sticky odorous compounds from an opening located in the snout. It is easy to think of nasute soldiers as nozzle heads discharging glue, but in reality what Rhynchotermes discharge is a strand, not fluid. The idea behind this is to turn your enemy into a sticky mess and incapacitate it. This is effective in case of attacking ants, perhaps termites’ worst enemies. The chemical properties of the compounds may also have a role in disrupting the ants’ chemical communication. Sometimes during the interaction the termite soldiers stick to the ants as well, sacrificing themselves for the benefit of the colony. But what if this does not work? Then they can use their secondary weapon – the mandibles.

*This image is copyright of its original author

The mandibles are curved (similar to those found in army ant soldiers) and double-hooked. I cannot help seeing them as reminiscent to the mandibles of young Epomis larvae. This is probably an adaptation to grab and hold on tight to whatever the termite is biting. I even tested it – not only the soldiers grab well, they also lock themselves in place. They are difficult to pull out, like a fishhook.

*This image is copyright of its original author

Another thing I noticed is that many soldiers had “broken noses”. I wonder if the snout has a breaking point to allow for a quick release of the gland’s contents onto the intruder. They too moved about clumsily looking for troublemakers to the colony, reminding me of a drunken guy trying pick a fight in a bar, broken bottle in hand.

*This image is copyright of its original author


*This image is copyright of its original author

There is still much we do not know about Rhynchotermes. For example, in the case of Rhynchotermes perarmatus, the alate caste was described only recently. Some Rhynchotermes species tend to occupy abandoned nests of other termites, but occasionally they are also found in close proximity to active nests, bordering the neighbouring colony or right on top of it. It would be interesting to examine what kind of interaction they have with other termite species. Like a lot of things in nature, these termites do not conform to our neat labels. Their bizarre soldiers represent the best of both worlds. They serve as a reminder that nature is full of surprises, that rules are meant to be broken, and that you do not have to look hard to find something new and inspiring.
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Today’s Termites Offer Clues to Ancient Migrations Across Southeast Asia: By Entomology Today.

Researchers at Universiti Sains Malaysia analyzed the genetic markers in more than 200 populations of the subterranean termite Macrotermes gilvus across Southeast Asia to piece together the paths along which the termites (and likely other terrestrial animals) spread throughout the region during the last 2 million years. (Photo credit: Chow-Yang Lee, Ph.D.)

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The subterranean termite Macrotermes gilvus is widespread across Southeast Asia, and has been throughout modern times. But, absent today’s human-aided transport of species across the globe, how did such a ground-dwelling insect get from mainland Myanmar all the way out to the islands of the Philippines, Indonesia, and Malaysia?


The answer lies in ancient land bridges that existed in eras when sea levels were lower, and researchers from Universiti Sains Malaysia (USM) say the genetic markers present in M. gilvus termites throughout varying Southeast Asia locales reveal the routes these termites—and many other terrestrial animals, as well as early humans—likely followed as they dispersed there over the past 2 million years.

USM’s G. Veera Singham, Ph.D., Ahmad Sofiman Othman, Ph.D., and Chow-Yang Lee, Ph.D., chose to analyze M. gilvus because it is currently widespread and because its regular dispersal flight range is short. Also, when it establishes new colonies, it depends on a particular soil structure and the availability of a fungal symbiont. “Therefore, the genetic signature of this species should reflect historical patterns rather than contemporary gene flow between different regions of Southeast Asia,” says Veera Singham.

The researchers collected termites from more than 200 populations of M. gilvus in regions across Southeast Asia and examined the genetic relationships between them using both mitochondrial genes and microsatellite markers.

In results published last week in PLOS ONE, Veera Singham, Othman, and Lee report that their phylogeographic analysis suggests a north-south dispersal corridor from Indochina to Java that existed between 1.09 million and 420,000 years ago, followed by a west-east dispersal route that existed between 870,000 and 340,000 years ago that allowed the termites to expand from the mainland toward the Philippines.

In a study of genetic markers in populations of the subterranean termite Macrotermes gilvus across Southeast Asia, researchers at Universiti Sains Malaysia found evidence for a dispersal corridor from Indochina to Java that existed between 1.09 million and 420,000 years ago (left) and a later dispersal event between 870,000 and 340,000 years ago in which the termites spread east to the Philippines (right). (Image credit: Veera Singham, et al, PLOS One, 2017)

*This image is copyright of its original author

“Termites would have spread through these routes on their own in the ancient times, but later in the modern times their dispersal could have further aided by human activities,” says Lee. “The termite dispersal routes are indicative of the presence of suitable habitable environment during the glacial periods that were also suitable for other animals (such as mammals) and including early humans to exploit similar routes for migration and movement.”

More here: “Phylogeography of the termite Macrotermes gilvus and insight into ancient dispersal corridors in Pleistocene Southeast Asia”
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For Termites, Home is Where the Molt Is: by Entomology Today.

As Formosan subterranean termites (Coptotermes formosanus) begin to molt, their outer exoskeletal layer is shed from the body, leaving a new one behind, as can be seen in some of the termites above. Research at the University of Florida has found that termites consistently return to their central nest to molt—a behavior that can be put to use in termite management efforts. (Photo originally published in Kakkar et al., Journal of Economic Entomology, September 2017)

*This image is copyright of its original author

If, like some creatures on this Earth, you had to periodically shed your skin, would you prefer to do that out in public or in the privacy of your own home?

If you’d choose the latter, then congratulations—you have at least one thing in common with a termite.

In a study published the Journal of Economic Entomology in September, researchers at the University of Florida found that worker-caste Formosan subterranean termites (Coptotermes formosanus) have a strong urge to return to their central nest when it’s time to molt. This habit was not previously understood, according to the study’s lead author Garima Kakkar, Ph.D., given the difficulty of studying termites’ underground behavior, and it’s also an advance in understanding why baits using chitin synthesis inhibitors (CSIs) have proven successful in eliminating termite colonies.

“Because CSIs kill termites during ecdysis [molting], our finding suggests that CSI-affected termites die in the central nest near the reproductives and brood,” Kakkar says. “This would prevent termites from dying near a bait stations that may result in bait aversion.”

Kakkar and her advisor, Nan-Yao Su, Ph.D., constructed a nest habitat for a Coptotermes formosanus colony that included both a central nesting area as well as foraging sites connected to the central nest by 15 meters of coiled tubes.

A diagram shows the habitat created for observation of a colony of Formosan subterranean termites (Coptotermes formosanus) by researchers at the University of Florida. A planar arena (60 × 60 × 0.9 centimeters in thickness) was filled with moistened sand and extended 15 meters in one direction through Tygon tubes. Three small planar arenas (24 × 24 × 0.6 centimeters in thickness) filled with moistened sand were attached at every 5 meters along the linear foraging distance. (Image originally published in Kakkar et al., Journal of Economic Entomology, September 2017)

*This image is copyright of its original author

After the colony had a full week to settle in to its habitat, the researchers collected and marked the location of 30 molting and 30 non-molting workers. While non-molting workers were found both in the central nest and in the foraging sites, all molting workers were found in the central nest. Specifically, they were near the colony’s eggs, no more than 5 centimeters away. And newly molted workers (within 36 hours post-molt) tended to stay in the central nest area, too.


Researchers in Su’s lab have been refining CSI baits since their inception about 20 years ago, and speeding up their efficacy is one goal. CSIs specifically work by killing the termites during their molting process; so, as long as CSI-baited termites still obey their compulsion to molt in the central nest, any formulations that would accelerate that process should remain effective.
“We hypothesize that molting workers in a colony would still exhibit the molt-site fidelity when baited with CSI. In a follow-up study, we have tested if workers would die in central nest when attempting to molt under the effect of CSI. That publication is in the pipeline,” Kakkar says.

In the current study, a large portion of non-molting workers were found in the nest area, as many of them are tasked with nest maintenance, grooming eggs, and feeding larvae. Previous research, meanwhile, has shown that an average of 1.7 percent of termite workers in a colony molt each day. The discovery that foraging workers return to the nest to molt suggests that they might trade roles with workers in the nest, Kakkar and colleagues write. They also speculate that the reasons for returning to the nest to molt could include simply seeking safety during a vulnerable time to feeding the queen the workers’ shedded layers (exuviae), which contain much-needed nitrogen.

“We are also currently testing a couple hypotheses to examine its biological significance—i.e., why do they have to go back to central nest to molt?” Kakkar says.
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Argentina Tshokwane Offline
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Termite soldiers.

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Harvester ants farm by planting seeds to eat once they germinate: By Vedrana Simičević

Dyed germinating seeds were fed to larvae in the laboratory, causing them to appear pink

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They’ve cracked it. Small ants carry home large seeds to eat all the time, but no one knew exactly how they managed to break through the seeds’ tough exterior.


It turns out that Florida harvester ants, Pogonomyrmex badius, have developed a clever farming strategy to do so – they plant seeds, wait for them to germinate and then eat the soft spoils.

Some 18 genera of ants harvest seeds, and colonies of some species can store more than 300,000 seeds in their underground granaries.

So far, scientists thought that ants must be able to break the seeds open and just ate them as they were. “The reality is a lot more interesting,” says Walter R. Tschinkel at the Florida State University.

“There are many studies of seed choice by forager harvester ants, but none of the authors asked the question of whether the ants can open the seeds,” says Tschinkel. “This may be in part because most of these studies were done on western harvester ants whose deep nests are in hard soil, so the seed chambers are not easily excavated.”

Seeds dyed different colours according to their size glow under ultraviolet light

*This image is copyright of its original author

With his team, Tschinkel excavated and studied approximately 200 P. badius nests and found that the ants mostly open and consume small seeds, which are easier to crack. Foragers collect seeds of all sizes, so this leads to the accumulation of larger seeds, which end up forming 70 per cent of stored seeds by weight.


In a series of lab and field experiments Tschinkel and colleagues showed that P. badius doesn’t seem to be able to open the large seeds unless they have germinated first. Even the caste of ants with large heads and mandibles thought to be specialised for seed opening can’t crack the big seeds.

Germination, on the other hand, splits the tough husk, making the seed contents available as food for the ants. A single large seed may have nutritional value of 15 smaller seeds, so it makes sense to collect it and wait for it to crack open. Seeds from various species germinate at different times, which may give the ants a steady supply of their “crop”.

This is the first example of ants relying on germination to consume large seeds, although some worms seem to do it, too. The only other example of ants farming plants for food is of the Fijian ant Philidris nagasau, which grows Squamellaria plants and harvests their fruit.
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Vertical organization of the division of labor within nests of the Florida harvester ant, Pogonomyrmex badius: By Walter R. Tschinkel , Nicholas Hanley.

Abstract

In the Florida harvester ant, Pogonomyrmex badius, foragers occur only in the top 15 cm of the nest, whereas brood and brood-care workers reside mostly in the deepest regions, yet the food and seeds foragers collect must be transported downward 30 to 80 cm to seed chambers and up to 2 m to brood chambers. Using mark-recapture techniques with fluorescent printer's ink, we identified a class of workers that ranges widely within the vertical structure of the nest, rapidly moving materials dropped by foragers in the upper regions downward, and excavated soil from deeper upward. Within the nest, only 5% of foragers were recovered below 20 cm depth, but about 30% of transfer workers and 82% of unmarked workers were found there. Below 70 cm depth, 90% of workers were unmarked, and were probably involved mostly in brood care. During the summer, the transfer workers comprise about a quarter of the nest population, while foragers make up about 40%. Workers marked as transfer workers later appear as foragers, while those marked as foragers die and disappear from the foraging population, suggesting that transfer workers are younger, and age into foraging. The importance of these findings for laboratory studies of division of labor are discussed. The efficient allocation of labor is a key component of superorganismal fitness.

Introduction

Division of labor is a hallmark of insect sociality, and is expressed along multiple axes. In social hymenoptera, the defining division is between reproductive (queen) and non-reproductive (worker) individuals, but specialization within each of these castes also occurs. Within the worker caste, division of labor is almost universally associated with worker age (age polyethism), and in those species with highly size-variable workers, with body size. Division of labor has been reviewed a number of times [1,2,3,4] and subjected to several theoretical or modeling treatments [1, 5–7]. In age polyethism, young workers are associated with brood care, moving to more general nest tasks as they age, and becoming foragers outside the nest in old age. These functional transitions have been described in dozens of species of ants (for more recent examples: [5,6]). In addition to changes in behavior, age polyethism is associated with a number of physiological, developmental, sensory and genetic changes. Gene activity changes [7], as do hormone levels [8], ovarian development [9,10], lipid stores [11] and responsiveness to task stimuli [12,13]. Occasional examples of the absence of age polyethism also exist [14,15]. A perennial question has been the degree to which division of labor and task selection are flexible or causally associated with age [7,8]

Unfortunately, most division of labor studies in ants have been carried out in artificial, simple laboratory nests that bear little or no resemblance to natural nests, or to natural ecological conditions. Laboratory studies have therefore usually missed two important aspects of division of labor. First, with a few exceptions (see below), laboratory studies are insensitive to the spatial organization of division of labor, that is, that shifting worker roles depend upon movement among particular parts of natural nests. And second, in nature, age-related transitions between worker roles are regulated by mortality of foragers, but such mortality is artificially lower in the laboratory. These two issues are described below.

Division of labor is organized within nest space

Porter and Jorgensen [16] and MacKay [17] showed that within their nests, several species of harvester ants are vertically stratified by age, with young workers and brood deep in the nest, and foragers and defenders in the upper regions. Tschinkel reported a similar distribution in P. badius [18] and in Prenolepis imparis [19]. In all of these, the young workers deepest in the nest had the highest fat content and dry weight, and the lowest respiratory rate, whereas the foragers in the upper regions had the lowest fat content [16,17,18,19,20,21] creating additional correlations. These studies suggest that as workers age, they move upward and change behavioral and physiological roles, finally foraging outside the nest as the last act of their lives. This adds a physical dimension to the age-related changes in worker behavior and physiology.

Workers of some roles seem to be more strongly segregated than others. Most obvious are foragers, as they are always found in the upper nest region [16,17]. The sharpness of this limitation was revealed through mark-recapture in P. badius by Kwapich and Tschinkel who found that foragers were completely limited to the top 15 cm of the nest [22,23]. A similar segregation of workers was found between fungus garden workers and trash workers in the leafcutter ant, Atta cephalotes [24], a segregation that reduces the introduction of pathogens into the nest [25].

In contrast to the majority of age polyethism studies, a few laboratory studies included a reasonably realistic spatial component. For the tiny colonies of Leptothorax albipennis and L. unifasciatus a laboratory nest between plates of glass may not be radically different from their natural nest in the thin spaces between stones. In such nests, workers of various roles are segregated in space, and resume that organization after forced re-nesting [26]. Other laboratory studies also showed spatial fidelity associated with division of labor [27,28]. The latter but not the former tested the effect of worker age, but neither revealed the degree to which the laboratory nest resembled the natural nest.

Extrinsic forager mortality shapes division of labor

Kwapich and Tschinkel found that most forager mortality resulted from extrinsic causes, and that whereas forager lifespan was only about 3 weeks in nature, it was many months in the laboratory [23]. In other words, in nature, foragers do not die of old age. Evolution over many generations has thus tuned colony demography to replace foragers at a rate determined by the average extrinsic mortality rate. An experimentally increased loss of foragers was not met with replacement beyond the demographic rate, but reduced forager mortality (by penning focal or neighboring colonies) inhibited the transition of younger workers into the foraging class. This implies that age-related division of labor is driven, in part, by extrinsic forager mortality, a factor that is almost absent in the laboratory. Whether transitions between other worker roles are also affected by forager mortality rate is unknown.

This paper is not so much about age polyethism as it is about identifying the component parts of the superorganism and how they are organized to carry out the various necessary functions. Because foragers never venture far below the nest surface, and the brood and their caretakers reside overwhelmingly in the deepest nest regions, there should exist an intermediary worker class to transport food and other material between these non-contacting groups. We present evidence that such a distinct class of transfer workers operates in the Florida harvester ant, P. badius, and that its operation is associated with worker age and location. We also argue that its transition to foraging is controlled by forager mortality.

Materials and methods

Study site

The study population of Florida harvester ant, Pogonomyrmex badius, is located in a 23 ha site (latitude 30.3587, longitude 284.4177) about 16 km southwest of Tallahassee, Florida, USA, within the sandhills portion of the Apalachicola National Forest. The site, Ant Heaven, consists of excessively drained sandy soil occupying a slope to a wetland and stream, causing its water table to be depressed (>5 m at the maximum), thereby making it suitable for several drought-resistant species of plants and animals. The forest consists of longleaf pines (Pinus palustris), turkey oak (Quercus laevis), bluejack oak (Quercus incana) and occasional sand pines (Pinus clausa) and sand live oak (Quercus geminata). The natural ground cover consists of several successional grasses and forbs, along with clonal shrubs such as shiny blueberry (Gaylussacia dumosa) and gopher apple (Licania michauxii).

This study was carried out under US Forest Service, Apalachicola National Forest permits APA583 and APA56302. No protected species were involved.

Fluorescent marking procedure

Marking objects and ants with fluorescent inks of different colors in order to recapture and distinguish them later was a central technique of this study. Using fluorescent printers ink dissolved in ether and sprayed on objects as a fine mist was first reported by Porter [29]), and was later adopted and further developed by Tschinkel [30] and Kwapich and Tschinkel [22] for mark-recapture estimation of ant forager populations. Printers ink in several fluorescent colors (green and orange were most commonly used) was purchased from Gans Co. (http://www.gansink.com/locations.asp) and diluted 1 part ink in 9 parts diethyl ether. This was sprayed on ants or seeds in a tray using a 5 mL, plastic perfume mister (Freund Container and Supply, freundcontainer.com), and allowed a few minutes to dry. Most colors were not easily seen in visible light, but shone like beacons under ultraviolet (Fig 1). There was no effect on ant mortality or behavior, and the ants handled marked seeds like unmarked seeds.

Fig 1. Marking with fluorescent printers ink and rhodamine dye.

A. a forager under UV light; B. seeds under visible light; C. the same seeds under ultraviolet light; D. ant larvae fluorescing after consuming rhodamine B-dyed food.

*This image is copyright of its original author

The fluorescent, vital, water-soluble dye, rhodamine B was used to dye pieces of tenebrionid beetle larvae (Zophabas atratus) which, when offered to P. badius colonies, were fed to ant larvae, causing them to fluoresce brightly under UV light (Fig 1D). This allowed the detection of the transport and ingestion of the pieces of beetle larvae.

Fluorescent marking of foragers

Each trial began with exhaustive marking of foragers [22]. Foragers were collected by baiting with seeds and cookie crumbs at least 1 m from the nest entrance. Foragers were defined as those workers that picked up bait and headed back to the nest. These foragers were captured and spray-marked with green fluorescent printers ink dissolved in ether (see above). Using the same color ink, this was repeated over several days, until 80% to 90% of foragers coming to baits were marked (one nest was only 75% marked).

Kwapich and Tschinkel [22] reported that fluorescent-marked foragers were found only in the top 15 cm of the nest, but that even when almost all foragers coming to baits were marked, excavation of the top 15 cm revealed that 54% of workers there were unmarked (i.e. were not foragers). It thus seemed likely that these unmarked workers in the upper parts of the nest potentially functioned as "transfer workers " (hereafter referred to as such) that traveled up and down in the nest moving seeds, food, soil and brood. The focus of this paper is on the identity and movement of these possible transfer workers. An additional hypothesis is that these unmarked workers are soon to become foragers, but the two hypotheses are mutually compatible.

Fluorescent marking of transfer workers

While foragers can be marked without disturbing the nest, transfer workers cannot, yet if the movements of marked transfer workers in the nest are to be studied, the upper zones of the nest disturbed during the capture of transfer worker must be restored to a reasonable facsimile of their original state. This was done as follows, once most of the foragers had been marked.

One day after forager marking was complete, the top 15 cm of the nest was excavated by sequentially exposing chambers with a brick trowel and lifting off chamber ceilings (as described in [18,22]). After collecting all workers from the exposed chambers, they were inspected under a UV light in order to separate green-marked foragers from unmarked workers. The unmarked workers were marked with orange fluorescent ink. Most of these "non-foragers" were what we provisionally designated as transfer workers, but also included the 9% to 20% of foragers that remained unmarked. These were then not distinguishable from transfer workers later in the study. We did not expect to recover all marked foragers in the top 15 cm because the nest was open and some foragers were afield.

Reconstruction of the nest

When each chamber had been exposed and emptied, a piece of transparent acetate film was placed over it and its outline traced. These outlines were transferred to a sheet of 1.3 cm thick foam insulation panel and then cut out to create a facsimile of the chamber. After adding a ceiling of acetate film held on with nails, the chamber-facsimile was buried at the same depth as the original chamber, covered with sand and provided with a connecting shaft to the chambers below and above. In this way, two to four real chambers were replaced with facsimiles of the same area and outline (Fig 2).

Fig 2. Examples of chamber facsimiles and their use by the ants in the reconstructed nests.

The foam board has been removed in the right panels, showing chamber contents. The non-chamber area in the right images has been digitally darkened to increase the visibility of the occupied chamber area. Note the brood (mostly pupae) in the right panels, and the sand from deeper levels deposited in the lower right chamber. Although brood can sometimes be found in the warmer, near-surface chambers, they compose only a small fraction (<2%) of a nest's total brood; data from [32].

*This image is copyright of its original author

Nest excavation and evaluation

Upon completion of the reconstructed nest, all workers, brood and other captured contents were released in an enclosure surrounding the nest entrance. The enclosure was removed in 24 hr or less. Two to six days later, colonies were offered fluorescent-marked seeds and tenebrionid larva pieces dyed with rhodamine B. Earlier work [22] had shown that such food items are quickly retrieved into the nest and transported downward, presumably by a class of workers other than foragers, that moves up and down in the nest. One to three hours after baiting, the vertical distribution of marked foragers and transfer workers within the nest was determined through chamber by chamber excavation and capture, following the procedures of Tschinkel [18] and Kwapich and Tschinkel [18,22]. Contents of each chamber were kept separate and were checked for fluorescent marks under UV in the laboratory. Fluorescent items scored included green-marked foragers, orange-marked transfer workers, seeds, pieces of dyed beetle larvae, fluorescing larvae and callow workers that had eaten the dyed tenebrionid larvae (Fig 1D). This procedure was applied to 11 colonies during the active season in 2016 and 2017.

Post-excavation procedure

After census and evaluation of nest contents in the laboratory, we created a subterranean nest in the colony's original location using ice [31], and released the colony into this nest after the ice melted. All colonies readily moved into these nests. At various elapsed times up to 50 days, foragers were captured as above and checked for green and orange-marked workers in order to evaluate forager survival and turnover.

Data analysis and availability

Data were graphed and analyzed by ANOVA, t-test and regression using Statistica 13 (Statsoft Inc.). Data are available in S1 Table and S2 Table.

Results

Use of facsimile chambers

All colonies used the facsimile chambers that replaced the destroyed chambers in the top 15 cm of their nests (Fig 2). The contents and activity of the facsimile chambers were similar to those of natural nests [18] [22]. In several cases, sand excavated from lower in the nest was deposited on the floors of the facsimile chambers (Fig 2B and 2D), as it often is in natural nests as well [18]. Relatively normal use of the facsimile chambers is a necessary condition for the subsequent assessment of marked-worker distribution within the nest.

Recovery of marked workers

Forager marking proceeded for a variable number of days. In eight experimental colonies, 80% to 91% of foragers were marked in three to five days, but two colonies required non-sequential marking efforts for 11 to 12 days to reach this criterion, and one 45 non-sequential days. The total number marked was referred to as the cumulative number of marked foragers. One day after forager marking was complete, the top 15 cm of the nest was excavated and all unmarked workers were marked as transfer workers (orange). All workers were then returned to the reconstructed nest. Because colonies were actively foraging during this procedure, only about 54% (s.d. 18%) of the cumulative marked foragers were recaptured in the top 15 cm. When adjusted for a mean of 14% of foragers that were unmarked, this value was about 62%.

Two to six days were allowed for the colony to occupy and adjust to the facsimile chambers. Just before excavation, colonies were offered seeds and dyed beetle larvae. One to three hours later, all workers on the surface were collected, followed by excavation and collection of all nest contents, chamber by chamber. Overall, 71% (s.d. 23%) of the marked transfer workers were recovered in the final nest excavation, but only 39% (s.d. 19%) of foragers were recovered. Lower forager recovery resulted from two factors—many were probably afield when the nest was excavated, and about 4% per day had died while foraging [22,23]. Given two to six days elapsing between completion of marking and final nest excavation, this amounted to a loss of 8% to 22% of foragers, respectively. Similar losses were also incurred during the more extended marking periods, but were difficult to estimate. There was a trend for lower forager and transfer worker recovery with longer elapsed times, but this was not quite significant. In any case, the central question of this paper does not require recovery of all marked workers because the measure of interest is their relative distribution within the nest.

Recapture on the nest surface

Because foragers are by definition active outside the nest, and because baiting and excavation were necessarily carried out while the colony was actively foraging, a large proportion of the total foragers recovered during final excavation were captured on the surface (Fig 3A). Fewer than half as many transfer workers and almost no unmarked workers were captured there (Fig 3A; F 2,27 = 13.95; p<0.00001). A similar proportion of transfer workers occurred below 20 cm (Fig 3B). In contrast, foragers were essentially absent below 20 cm (Fig 3B), a region in which most workers were unmarked.

Fig 3.

Capture of workers on the nest surface (A), and deeper than 20 cm in the nest (B). Marked foragers were captured primarily on the ground surface and were essentially absent below 20 cm depth. Transfer workers occurred in both regions, and unmarked workers primarily deep in the nest.

*This image is copyright of its original author

Vertical distribution of workers within the nest

Of greatest interest for this study is how the marked and unmarked workers recovered underground distributed themselves in the vertical space of the subterranean nest. All foragers were originally captured and marked on the surface, and all transfer workers were originally captured and marked in the top 15 cm of the nest. Does either class move up and down in the nest with significant frequency? If so, they would fulfill one of the requirements for transfer workers. Because colonies differed greatly in population size, the vertical distribution of these worker classes was expressed as percent of each group in each 10 cm increment of nest depth.

Surface capture is distinguished from forager baiting by taking place on the mound surface without baits. Fig 4A shows that about 95% (s.e. 1.6%) of ants marked as foragers were recovered either on the surface (55%) or in the top 20 cm of the nest (40%). A mean of only 5% (s.e. 1.6%) of foragers occurred below 20 cm. In other words, foragers were predominately recaptured in the zone in which they were captured in the first place. On the other hand, marked non-foragers (presumed transfer workers) first captured in the top 15 cm of the nest did not remain there, but were spread much more evenly across the nest's vertical structure. About 30% (s.e. 5.4%) were recovered below 20 cm, with some as deep as 110 and 120 cm, the depth at which larvae normally reside. The recovery of a large number of transfer workers on the surface (about 25%) suggests several possibilities: (1) they were foragers that were mismarked as transfer workers (about 14% of foragers), (2) they were sand workers whose role was to take out excavated sand, trash, etc. These are intermediate in age, and later transition into foragers[33]; (3) they were transfer workers transitioning directly into foragers. These transitions remain to be well-characterized.

Fig 4. Distribution of marked and unmarked workers within the nest.

Depth is shown in 10 cm increments. A. percent of the total of each type recovered; B. percent of each type chamber by chamber. Photo on the left is an aluminum cast of an approximately average P. badius nest.

*This image is copyright of its original author

Unmarked workers increased steadily with depth, with 82% (s.e. 2.8%) occurring deeper than 20 cm (Fig 4A). Below 70 cm, they made up over 90% of the workers.


Because the subterranean distribution of transfer workers is the primary focus of this study, Fig 4B shows the chamber-by-chamber ratios of the three worker classes. Whereas foragers were essentially absent from chambers deeper than 20 cm, transfer workers were found below that depth, and continued to make up a significant proportion of the workers in chambers. Below about 70 cm, transfer workers made up a mean of about 6% (range 0.04% to 10.3%) of the workers, with unmarked workers making up the difference (mean 94%). The occurrence of workers that had been marked in the top 15 cm at such nest depths suggests that transfer workers, in contrast to foragers and unmarked workers, are vertically mobile.

Downward transport of food

The apparent vertical mobility of transfer workers is supported by the rapid movement of seeds and beetle larva pieces deep into the nest, a movement unlikely to be carried out by foragers because these are essentially absent below 20 cm. During the one to three hours elapsing between the offering of fluorescent-marked seeds and dyed beetle pieces, foragers collected these items and deposited them in the top 15 cm of the nest [26]. During the subsequent nest excavation, these items were found at all levels of the nest, far deeper than their original locale of deposition (Fig 5). The total marked seeds brought into the nest ranged from 17 to 294, and of dyed tenebrionid pieces, 2 to 30. The presence of between 5 to 258 fluorescing ant larvae, and 14 to 180 fluorescing callow workers showed that the dyed pieces had actually been eaten. Two colonies even contained 6 and 7 fluorescing pupae.

Fig 5. Distribution of marked seeds, larvae, callow and beetle pieces by depth.

A. Distribution of marked seeds in 10 cm increments of depth; B. Distribution of fluorescing larvae and callows in quarters of maximum nest depth. Fluorescence resulted from feeding on dyed beetle pieces; C. Distribution of dyed beetle pieces in quarters of maximum nest depth. Eight nests were offered beetle pieces, and 2 to 30 pieces were recovered in the nest. Photo on the left is an aluminum cast of an approximately average P. badius nest.

*This image is copyright of its original author

Fluorescing seeds were most abundant in the top 20 cm of the nest, reflecting their rapid deposition in this zone by foragers (Fig 5A). No marked seeds made it deeper than about 60 cm reflecting the depth of most seed storage chambers (between 30 and 80 cm) (Fig 6A). This is also the zone in which transfer workers made up 20% to 40% of workers in chambers, whereas at depths greater than 50 cm, they made up 0 to 10%. This pattern suggested that a large proportion of transfer workers moved seeds into storage chambers, as seems reasonable for a seed-harvesting ant. Fewer serviced the brood chambers located at greater depth than seed chambers (>80 cm) (Fig 6A).

Fig 6. Distribution of chambers by type and nest depth.

A. Seed and brood chambers as a percent of the total; B. Total chambers.

*This image is copyright of its original author

In contrast to seeds, fluorescing larvae and callows were most abundant in the deepest regions where 98% of brood are normally kept [34] (Fig 4B; Fig 5B; Fig 6A). The fluorescence was derived from feeding on dyed beetle-larva pieces, the majority of which were recovered between 0 and 60 cm, with about 12% deeper (Fig 5C). Not all dyed pieces had been eaten, but clearly enough had to cause a large number of ant larvae and callows to fluoresce.

Colony composition by worker roles

What fraction of the colony performs each of the three major roles, forager, transfer worker and unmarked (probably younger) worker? These values were approximated as follows: (1) the forager population was estimated from the cumulative number marked and the final percent marked, discounted for forager mortality of 4% per day for the days elapsing between the last mark day and final nest excavation. This discount ranged from 8% to 22%; (2) the estimated unmarked foragers were added to the forager estimates; (3) transfer workers marked during the excavation of the top 15 cm were counted directly; (4) the unmarked foragers were subtracted from the transfer worker counts because unmarked foragers were almost certain to be in the top 15 cm of the nest. They were, of course, mis-marked with the transfer worker color (orange).

These estimates yielded the total ants in each colony and the percent of workers in each role. Colonies ranged from about 500 to 4500 workers (mean 2000, s.d. 1250). Foragers averaged 41% (s.d. 6%) of the colony, transfer workers 23% (s.d. 13%) and unmarked 36% (s.d. 14%). Transfer workers are thus a minority of the workers, but together with unmarked workers make up almost 60% of the colony. Our mid-summer estimate of the percent foraging is somewhat higher than the more precise mid-summer peak of about 35% reported by Kwapich and Tschinkel [22], but unlike theirs, ours was independent of colony size (p> 0.5; n.s.). On the other hand, the fraction of transfer workers decreased with colony size (fraction transfers = 0.35–0.000059* total workers; p<0.08; R2 = 31%) while the fraction of unmarked workers increased at a similar rate (fraction unmarked = 0.23+0.000068* total workers; p< 0.05; R2 = 36%).

Post-excavation turnover in marked foragers

If transfer workers are ageing into foragers, and foragers are dying on the job, then ants bearing the forager mark (green) should gradually fade from the foraging population while ants bearing the transfer worker mark (orange) should increase, as should unmarked workers. This is just what happened when foragers were assessed 20 or 50 days after the colony was replanted in its original location. The proportion bearing the green forager mark decreased from about 79% (s.d. 15%) on the final day of forager marking to 12% (s.d. 6) after 20 days and 7% after 50, days. At 50 days, three of six colonies had no green-marked foragers at all. At the same time, the proportion bearing the orange transfer worker mark increased from 0% at the time of initial marking to 41% (s.d. 13%) at 20 days and 42% at 50. At the same time, unmarked foragers increased from about 14% (s.d. 6.1%) to 46% and 52%.

Discussion

Division of labor and worker age in the Florida harvester ant are both strongly associated with location within the vertical nest structure, suggesting that there is an essential spatial element to division of labor. Foragers occur only in the uppermost chambers of the nest, where they deposit items brought in from the field [16,22], while the deepest regions of the nest are home mostly to brood and young workers who act as nurses for the brood [34], although some brood may be found temporarily in the warmer upper nest regions. There seems to be little or no direct exchange between these two groups, as neither normally ventures far from their "home" zone. We have now shown that a third class of workers acts as shuttles, transferring items of food downward and excavated soil (and possibly brood under some circumstances) upward. These non-foraging, vertically-mobile workers have several of the characteristics expected of such shuttle or transfer workers—(1) they can be captured in the upper chambers where their distribution completely overlaps with that of foragers when these are within the nest, giving them access to foraged items; (2) a substantial fraction of them (>30%) moves between the upper chambers where they were initially captured and deeper regions; and (3) their redistribution correlates and coincides with the movement of seeds and other food items downward; (4) their gradual later appearance in the role of foraging, replacing foragers that have died suggests that they are of intermediate age between brood workers and foragers; (5) their intermediate age is also supported by their intermediate fat content and location in the nest [34].

As in other tasks undertaken by social insects, the labor of transfer workers is probably organized in a series-parallel manner [1]. Thus, items as diverse as excavated sand pellets, food, leaf fragments (in leafcutter ants), trash and brood are typically transported by caching [33,35,36,37,38]. It is unlikely that any individual transport worker carries items the entire distance. The vertical mobility of transport workers, combined with their series-parallel mode of working causes them to move seeds and soil by sequential caching [33]. One must thus imagine a population of workers that constantly moves up and down over small to moderate distances within the nest performing whatever transport tasks they come upon.

The parallel patterning of labor and age in space requires that workers have information on their location. This information could conceivably be social, with ants in particular roles arranging themselves with respect to other roles. This would require cues identifying roles. Social patterning has been suggested in the tiny nests of L. fasciculatus, with workers referencing their own location relative to other groups [26]. Unspecified, but possibly social cues organized the laboratory nests of Camponotus fellah [28] and Myrmica rubra [27]. A possible source of soil depth information was proposed by Tschinkel [18] who noted a logarithmic increase in carbon dioxide concentration with depth. Ants have specific sensillae that detect carbon dioxide [39] and are known to respond to this gas with altered behavior [40,41,42]. Sadly, experimental tests showed that P. badius workers do not use soil carbon dioxide gradients as a template to arrange themselves in vertical space or to construct depth-appropriate chambers [43]. The depth cues (or social cues) used by P. badius remain unknown.

Labor, space and age are thus all correlated, but causal links are obscure. It seems most likely that ageing results in physiological, sensory and neural changes that in turn cause workers both to change roles and location within the nest. Even in simple laboratory nests, roles are spatially segregated, as anyone who has kept pet ants can attest [27,28,44]. In P. badius, the upward movement with age is "deliberate," for when young and old workers were arranged evenly in a vertical nest, the older workers moved upward to return to the region from which they had originally been taken [18]. The upward movement is probably not a rigid movement from level to level, but one in which as workers age, they gain increased vertical mobility (in both directions) followed eventually by residence in only the upper chambers and finally foraging on the surface (Fig 7).

Fig 7. Schematic representation of the within-nest migration and movement of workers of changing roles and age.

Only foragers leave the nest and die soon after doing so. They never occur deeper than about 15 cm in the nest. Transfer workers shuttle between foragers in the top of the nest and seed chambers and brood deep in the nest, shuttling materials such as food, soil and brood. As they age, they transition into sand workers and then foragers, although foragers often also dump sand, suggesting overlap between these roles.

*This image is copyright of its original author

Our interpretation acknowledges the usually non-overlapping differentiation of labor into foraging and brood care, along with a rather vague, ill-defined, mobile and flexible transition between the two often referred to as "reserve" workers [45,46]. Our scheme is also akin to the "spatial fidelity zones" of L. fasciculatus [26], but differs from them primarily in scale and geometry—concentric millimeter-sized zones in L. fasciculatus and vertical zones stretching over meters in P. badius. Whereas the association of worker age with spatial fidelity zones was weak in L. fasciculatus, it is very strong in P. badius. Perhaps this is not surprising—the enormous dimension of the spatial fidelity zones in P. badius make the association of zone and age both necessary and obvious [16,22,34]. In both species, the spatial fidelity zones expand and contract seasonally, driven, no doubt, by seasonal demography.


Given the already apparent variation, it seems likely that the most basic worker movement is not necessarily upward in a vertical nest, as in P. badius, Pr. imparis [19] and F. japonica [21], but outward from the brood region, so that the geometry of movement differs by species and nest architectures. This links the movement in L. fasciculatus with that in the aforementioned species. In S. invicta, brood are kept in the region of optimal temperature, rather than simply the deepest part of the nest, and workers move outward to the nest perimeter and into foraging tunnels as they age [45,47]. Moreover, S. invicta moves brood rapidly in response to changes in temperature [48]. Pheidole morrisi also keeps brood in the warmest regions, changing locations with seasons[49].

We have thus differentiated another distinct role along the space-by-age axis. More may remain to be characterized. The distinctness or overlap among roles, and the nature and timing of transitions also remain to be elucidated. Is the shift from transfer worker to forager a gradual shift associated with experience or practice? After all, carrying out the dirt and trash requires much less skill than orienting and navigating during foraging. Perhaps transfer workers acquire the necessary foraging skills while dumping excavated sand on the surface, or doing midden and guarding work. In Formica rufa, inexperienced spring foragers gradually take on the characteristics of older "veteran" or "pioneer" foragers [50]. In P. occidentalis, foragers are positively phototactic and show a circadian behavioral and gene activity rhythm while brood care workers do not [51]. In M. rubra, foragers are positively phototactic while brood workers are negatively phototactic [27], a behavioral difference that may be widespread in ants. In honeybees, the transition from guard to forager is accompanied by short orientation flights. In this light, the increasingly complex behavioral capacities of ageing workers are accompanied by neural growth and increased neural complexity [52]. In principle, this question applies generally across ant species when "reserves" change roles into foragers.

Considering the ill-defined, flexible roles of reserves, these may actually be differentiated into multiple, possibly sequential roles [33,35], such as occurs in the fire ant, Solenopsis invicta—foragers are first recruits waiting in the nest or foraging tunnels, and then age into scouts searching for food on the surface, a role in which they live only 2–3 weeks [30]. Tschinkel et al. [33] found that some foragers of P. badius also dumped excavated sand, but non-foraging sand workers later became foragers, suggesting overlap between the two tasks. In a sense, transfer workers in P. badius may be the analogs of recruits or reserve workers, but rather than transporting items from the field into the nest, they transport items within the nest.

Demographic processes in the superorganism such as the transfer worker-to-forager transition are probably central to the efficient allocation of labor appropriate to season, colony size and other factors [22]. Our evidence that transfer workers age into foragers suggests that this is the transition shown to be sensitive to foragers mortality rate—reduction of forager mortality by penning colonies or their neighbors reduced the transition of younger workers (probably transfer workers) into foragers. Conversely, experimentally-increased forager mortality did not speed the replacement of the lost foragers [23]. The division of labor and its allocation are thus extrinsically controlled in one direction by reduced forager mortality, while the other direction is unresponsive to increased forager losses. This raises the possibility that this control echoes deep into the nest to affect the transition from brood care to transfer workers. This in turn suggests a caveat for future laboratory studies: the lower death rate of foragers in laboratory experiments is likely to produce artifacts in the division of labor.

Labor is a core resource for the superorganism, and like other resources such as energy and material, natural selection tends to optimize its allocation. Labor applied to one set of tasks is not available to others, so that particular patterns of allocation can be expected to maximize colony efficiency and therefore fitness. Moreover, optimal allocation may vary seasonally, with colony size and probably other factors. Our identification of transfer workers adds another element to allocation patterns already described for foragers and brood care workers within the superorganism.
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Credits to Ruth Spigelman‎.

Winged Bull ant, also known as Bulldog ant (Myrmecia sp.) 40mm length. These large, spectacular, alert ants have characteristic big eyes, long, slender mandibles and a potent venom-loaded sting.They have superior vision - this one was tracking me during the photo session from up to 1 metre away. Seen here against the glass of my kitchen door. I believe there are approximately 90 species of bull ants in this country....mid-coast New South Wales, Australia.

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Credits to Termite Research Team.

Hospitalitermes Holmgren, 1912 (Nasutitermitinae); a random snap from the forest in South China (Xishuangbanna, Yunnan). One of few termites to be seen freely, as it feeds on microepiphytes (lichens, algae, mosses), which forages from the trees, and sometimes it feeds also at fresh leaf-litter.

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( This post was last modified: 01-27-2018, 08:32 PM by Tshokwane )

Excellent documentary about Wood Ants.

David Attenborough is in the Swiss Jura Mountains to discover the secrets of a giant. Beneath his feet lies a vast network of tunnels and chambers, home to a huge empire of ants. It is believed to be one of the largest animal societies in the world, where over a billion ants from rival colonies live in peace. Their harmonious existence breaks many of the rules for both ants and evolution, and raises some important questions. Through winter, spring and into summer, David turns detective to find the answers.



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Credits to ScienceNews.

Ants practice combat triage and nurse their injured:
A Matabele ant from Africa uses her mouthparts to treat a nest mate’s wounded leg in a prompt and effective insect version of health care.

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No wounded left behind — not quite. Ants that have evolved battlefield medevac carry only the moderately wounded home to the nest. There, those lucky injured fighters get fast and effective wound care.


Insect colonies seething with workers may seem unlikely to stage elaborate rescues of individual fighters. Yet for Matabele ants (Megaponera analis) in sub-Saharan Africa — with a mere 1,000 to 2,000 nest mates — treating the wounded can be worth it, says behavioral ecologist Erik Frank at the University of Lausanne in Switzerland.

Tales of self-medication pop up across the animal kingdom. For Matabele ants, however, nest cameras plus survival tests show insects treating other adults and improving their chances of survival, he and colleagues report February 14 in Proceedings of the Royal Society B. For treatment boosting others’ survival, Frank says, the closest documented example is humans.

In Ivory Coast, Frank studied Matabele ant colonies that staged three to five termite hunts a day. He and colleagues at the University of Würzburg in Germany published research last year showing that members of a hunting party carry injured comrades home.

Leaving the carnage of a battle with termites, a Matabele ant from Africa carries an injured nest mate home for treatment.

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Frank took a closer look at rescues after he accidentally drove over a Matabele ant column crossing a road. Survivors “were only interested in picking up the ants that were lightly injured, and leaving behind the heavily injured,” he says.


When Frank later set injured ants in front of columns trooping home from raids, injured ants minus two legs typically got picked up. Only once did an ant with five missing legs get a lift.

Ants that have lost two legs still have value to a colony, especially in a species where only about 13 new adults a day emerge from pupae. Four-legged ants regain almost the same speed that ants have on six legs, he says. In a typical hunting party, about a third of the ants have survived some injury, but most ants have at least four legs left. 


How the ants triage a battlefield evacuation is shaped by the injured ants’ behavior, Frank says. Ants with only moderate injuries, such as two lost legs, emit “help me” pheromones. These ants tuck in their remaining legs and generally cooperate with the rescuers. Not so with ants more seriously hurt, who may not even give off pheromones. Rescuers still stop to investigate. But the seriously injured ants often flail around instead of cooperating, and the rescuers give up.

Frank also has seen ants act more severely injured than they truly are. If the returning fighters bypass them, “they will immediately stand up and run as fast as they can behind the others,” he says. “In humans, it’s a very selfish behavior.” For ants, predators lurk, and the colony benefits by finding the injured first.

For injured raiders that do get home, another ant — usually not the carrier — steps in to treat the wound by repeatedly moving her mouthparts over it. When Frank isolated the ants to prevent this wound licking, about 80 percent of injured ants died. When he allowed ants an hour of treatment before isolating them, only 10 percent of them died.


Based on Frank’s observations, others who study ants are now wondering if they also have seen such rescue tactics. Andy Suarez of the University of Illinois at Urbana-Champaign wants another look at big Dinoponera australis that he’s frequently seen prowling for prey despite missing a limb. And Bert Hölldobler wonders whether weaver ants he has seen retrieving injured nest mates after battle were rescuing them. The usual interpretation has been cannibalism, says Hölldobler, at Arizona State University in Tempe.

Frank, however, used bright acrylic spots to track the fate of rescued Matabele ants. They weren’t for lunch.

WOUNDED IN BATTLE 

Africa’s Matabele ants, which attack termites in violent battles, offer the first documented case of animals other than humans doing successful wound care and even battlefield triage. When a mildly injured hunter gets carried home, other ants increase her chance of survival by repeatedly “licking” the injured leg (first video clip). An ant with very serious injuries (second clip) at the combat site doesn’t cooperate with attempted rescues and isn’t carried home. A mildly injured ant can act more injured than she is (third clip). If ignored, she will stand up and run (fourth clip) after potential rescuers.



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Credits to eLIFE.

Study reveals how forager ants keep their colonies well fed:

Ants can adjust their foraging activities to match the hunger level of their overall colony through individual interactions with their nestmates alone, according to new findings published in eLife.


The study, from the Weizmann Institute of Science, Israel, sheds new light on how a natural cooperative system, which has no central control and in which each individual has only partial knowledge, can achieve regulation. This could offer insight on how we handle some of our own systems that rely on distributed control, such as mobile networks and the electricity grid.

Forager ants make up only about 10% of an entire colony. They collect food to feed their nestmates, facilitated by a ‘social stomach’ – a crop organ situated upstream of their main stomach. Food collected by a forager ant can either be transferred to the main stomach for digestion or regurgitated from the social stomach and shared with other ants through a kind of mouth-to-mouth process known as trophallaxis.

“The question is how such a small number of forager ants can assess the current nutritional requirements of their entire colony,” says senior author Dr. Ofer Feinerman of the Weizmann Institute. “How much information regarding the colony’s state must a forager have to be able to fulfill its task? For example, does an ant getting ready to leave for its next foraging trip need to know the level of hunger of each of its nestmates, or is it enough that it knows roughly how hungry the overall colony is?”

“ Open annotations (there are currently 0 annotations on this page). Feinerman and his team used two techniques to discover how the collective goal of colony satiation is achieved by single Camponotus sanctus ants, assuming that individual ants would not necessarily have full knowledge of this goal. Fluorescent imaging allowed the researchers to see into the stomachs of individual forager ants and to quantify how much food was in each. Barcoding the ants with tiny tags enabled them to identify each individual ant during the entire feeding process.

As the colony members ate, the scientists watched the flow and storage of food among them and identified microscopic behavioural ‘rules’ followed by foragers that dictated the rates at which food flowed into the colony.

“We found that foragers deliver food at a rate that depends on the amount of food already stored in the crops of individuals they encounter,” says first author Dr. Efrat Greenwald, a postdoctoral fellow in Feinerman’s lab. “The amount of food that foragers pass to a single nestmate first seems quite random – the receiver is not filled to capacity and the forager does not empty its entire load. Nevertheless, there is a strong link between the amount of food passed to a nestmate and the amount already in that receiver’s crop. The receivers, we found, generally represent how hungry the colony is, and this suggests that food-flow rates are matched to the hunger level of the entire colony. Forager ants can therefore adjust their activities according to the colony’s overall hunger level without knowing explicitly what this is.”

“The next step will be to study the underpinnings of collective nutritional regulation in relation to more complex nutritional challenges, such as choosing between different food sources that vary in quality or composition. These are well known to occur in social insect colonies,” says co-first author Lior Baltiansky, a PhD student in Feinerman’s lab. “Looking more broadly to the field of distributed control, where the inner workings of some of the most common manmade networks are not fully grasped, we may have a thing or two to learn from ants, which are one of nature's exemplars of distributed workings.”
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Credits to Termite Research Team.

Termite of the Week


Orthognathotermes Holmgren, 1910 (Termitinae) je neotropický termit živící se na rozhraní shnilého dřeva a půdy. Vojáci mají nezvykle tvarované mandibuly, kterými mohou nejen kousnout, ale i lusknout.

Orthognathotermes Holmgren, 1910 (Termitinae) is Neotropical wood-soil interface feeder. Soldiers reveal unusually shaped mandibles that can be used for both, biting and snapping.

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