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ON THE EDGE OF EXTINCTION - A - THE TIGER (Panthera tigris)

Italy Ngala Offline
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Molecular cloning and functional characterization of eleven subtypes of interferon-α in Amur tigers (Panthera tigris altaica) Zhao et al., 2017

Highlights

• First cloning and characterizing of eleven subtypes of interferon-α in Amur tigers.
• Detailed biological activities of PtIFN-αs were described.
• Superior antiviral activities of PtIFN-α and PtIFN-α9 were proved.

Abstract:
"Interferon has a broad-spectrum of antiviral effects and represents an ideal choice for the development of antiviral drugs. Nonetheless, information about alpha interferon (IFN-α) is vacant in Amur tiger (Panthera tigris altaica), an endangered species and indigenous to northeast Asia. Herein, 11 PtIFN-αs genes, which encoded proteins of 164–165 amino acids, were amplified. Afterwards, expression and purification were conducted in Escherichia coli. In physicochemical analysis, PtIFN-αs were shown to be highly sensitive to trypsin and remained stable despite changes in pH and temperature. In feline kidney cells (F81)/vesicular stomatitis virus (VSV)/canine distemper virus (CDV)/avian influenza virus (AIV) systems, PtIFN-αs were demonstrated to have distinct antiviral activities, some of them (PtIFN-α and PtIFN-α9) inhibited viral transcription levels more effectively than the other subtypes including Felis catus IFN-α, an effective therapeutic agent used for viral infections clinically. Additionally, PtIFN-α and PtIFN-α9 can up-regulate the transcription and expression of p53, a tumor suppressor factor, which could promote apoptosis of virus-infected cells. In conclusion, we cloned and expressed 11 subtypes of PtIFN-α for the first time. Furthermore, PtIFN-α and PtIFN-α9 were likely to be more efficient against both chronic viral infections and neoplastic diseases that affect the Amur tiger population. It will be of significant importance for further studies to protect this endangered species."
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Messages In This Thread
RE: ON THE EDGE OF EXTINCTION - A - THE TIGER (Panthera tigris) - Ngala - 12-16-2017, 09:53 PM
Demythologizing T16 - tigerluver - 04-12-2020, 11:44 AM
Tiger Data Bank - Apollo - 07-28-2014, 09:54 PM
RE: Tiger Data Bank - Apollo - 07-28-2014, 10:02 PM
RE: Tiger Data Bank - Apollo - 07-29-2014, 12:56 AM
RE: Tiger Data Bank - peter - 07-29-2014, 07:05 AM
Tiger recycling bin - Roflcopters - 09-04-2014, 01:36 AM
RE: Tiger recycling bin - Pckts - 09-04-2014, 02:22 AM
RE: Tiger recycling bin - Roflcopters - 09-05-2014, 01:01 AM
RE: Tiger Data Bank - Apollo - 11-15-2014, 10:07 PM
RE: Tiger Data Bank - Apollo - 11-15-2014, 10:57 PM
RE: Tiger Data Bank - Apollo - 11-15-2014, 11:33 PM
RE: Tiger Data Bank - Apollo - 02-19-2015, 11:25 PM
RE: Tiger Data Bank - GuateGojira - 02-23-2015, 11:36 AM
Status of tigers in India - Shardul - 12-20-2015, 03:23 PM
RE: Tiger Directory - Diamir2 - 10-03-2016, 04:27 AM
RE: Tiger Directory - peter - 10-03-2016, 06:22 AM
Genetics of all tiger subspecies - parvez - 07-15-2017, 01:08 PM
RE: Tiger Predation - peter - 11-11-2017, 08:08 AM
RE: Man-eaters - Wolverine - 12-03-2017, 11:30 AM
RE: Man-eaters - peter - 12-04-2017, 09:44 AM
RE: Tigers of Central India - Wolverine - 04-13-2018, 01:17 AM
RE: Tigers of Central India - qstxyz - 04-13-2018, 08:34 PM
RE: Size comparisons - peter - 07-16-2019, 05:28 AM
RE: Amur Tigers - peter - 05-20-2021, 07:13 AM
RE: Amur Tigers - Nyers - 05-21-2021, 08:02 PM
RE: Amur Tigers - peter - 05-22-2021, 08:09 AM
RE: Amur Tigers - GuateGojira - 04-06-2022, 12:59 AM
RE: Amur Tigers - tigerluver - 04-06-2022, 01:08 AM
RE: Amur Tigers - tigerluver - 04-06-2022, 09:08 AM
RE: Amur Tigers - tigerluver - 04-06-2022, 11:30 PM
RE: Amur Tigers - peter - 04-08-2022, 07:27 AM



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